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Catalog Number | orb257262 |
---|---|
Category | Proteins |
Description | CD83 antigen is also known as B-cell activation protein and Cell surface protein HB15. CD83 is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD83 is expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. CD83 may play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 14.9 kDa |
Target | CD83 |
Protein Sequence | AAH30830.1 |
Source | Human CD83, His Tag (orb257262) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Ala 143 (Accession # Q01151-1). |
Expression System | HEK293 |
Expression Region | Thr 20 - Ala 143 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH30830.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD83,HB15,hCD83,BL11 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-18 kDa, 20-22 kDa and 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD83 protein
Unconjugated | |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide.The apparent molecular mass of CD83-hFc is approximately 35-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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