Human CD71 Mouse Monoclonal Antibody

SKU: orb1184691

Description

Human CD71 Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC, ICC
• Dilution range: ICC/IF=1:100-500, Flow-Cyt=1ug/test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, k
• Clone No.: 7B4
• Immunogen: KLH conjugated synthetic peptide derived from human Transferrin receptor
• Target: TFRC
• Molecular Weight: 95 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4).
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; TFRC; Transferrin receptor(p90 CD71); TRFR; TFR1_HUMAN.

Blank control: K562. Primary Antibody (green line): Mouse Anti-human CD71 antibody (orb1184691), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Mouse IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CD71) monoclonal Antibody, Unconjugated (orb1184691) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

TFRC

UniProt

P02786