You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb257279 |
---|---|
Category | Proteins |
Description | CD55 is also known as DAF or decayaccelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologicallygenerated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on nonforeign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection. |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 35.8 kDa |
Target | CD55 |
Protein Sequence | NP_000565.1 |
Source | Human CD55, His Tag (orb257279) is expressed from human 293 cells (HEK293). It contains AA Asp 35 - Ser 353 (Accession # NP_000565.1). |
Expression System | HEK293 |
Expression Region | Asp 35 - Ser 353 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000565.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD55,DAF,CR,CROM,TC Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.8 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Kwon, Young-Chan et al. Distinct CD55 Isoform Synthesis and Inhibition of Complement-Dependent Cytolysis by Hepatitis C Virus J Immunol, 197, 1127-1136 (2016)
SDS-PAGE analysis of Human CD55 protein
> 95% by SDS-PAGE. | |
KMP1396, Recombinant Human CD55/DAF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp35-Ser353) of human CD55/DAF (Accession #NP_000565.1) fused with a 6×His Tag at the C-terminus. |
Filter by Rating