Human CD45 Mouse Monoclonal Antibody

SKU: orb526577

Description

Human CD45 Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, k
• Clone No.: HI30
• Immunogen: Recombinant human CD45
• Target: PTPRC
• Molecular Weight: 180-220 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4).
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

B220; CD 45; CD-45; cd45 antigen; ec3.1.3.48; CD45R; GP180; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Ly-5 glycoprotein; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency.

Blank control: Molt4. Primary Antibody (green line): Mouse Anti-CD45 antibody (orb526577), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575