Human CD274 (B7-H1, PD-L1) Mouse Monoclonal Antibody

SKU: orb1172573

Description

Human CD274 (B7-H1, PD-L1) Mouse Monoclonal Antibody

Research Area

Autoimmune, Cancer Research, Cell Markers, Immunology

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4C11
• Target: CD274
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed cell death 1 ligand 1; CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; PD L1; PD1L1_HUMAN; PD1L1_Mouse; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Programmed death ligand 1; RGD1566211.

Blank control (black line): MDA-MB-231. Primary Antibody (green line): Mouse Anti-Human PD-L1 antibody (orb1172573), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

CD274