Human CD15 (SSEA-1) Mouse Monoclonal Antibody

SKU: orb1183962

Description

Human CD15 (SSEA-1) Mouse Monoclonal Antibody

Research Area

CD Markers, Cancer Research, Human Lineage-negative, Immunology, Stem Cell Research, Tumor Biology, cardiovascular Research

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1:50-100
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: 15F2
• Immunogen: Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4).
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1; Sialyl-Lewis X;

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (human CD15 (SSEA-1)) Monoclonal Antibody, Unconjugated (orb1183962) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (human CD15 (SSEA-1)) Monoclonal Antibody, Unconjugated (orb1183962) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.