Human C-Type Lectin Domain Family 7, Member A (CLEC7A) ELISA Kit

SKU: orb781398

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 7, Member A(CLEC7A).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLEC7A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLEC7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLEC7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLEC7A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.064 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BGR ELISA Kit, CLECSF1 ELISA Kit, BGR ELISA Kit, CANDF4 ELISA Kit, CLECSF12 ELISA Kit, DECTIN1 ELISA Kit, Beta-glucan receptor ELISA Kit, C-type lectin superfamily member 12 ELISA Kit, Dendritic cell-associated C-type lectin 1 ELISA Kit

Quick Database Links

UniProt

Q9BXN2