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Human Bcl2 Associated Death Promoter (BAD) ELISA Kit

Catalog Number: orb1949652

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1949652
CategoryAssays and Kits
DescriptionHuman Bcl2 Associated Death Promoter (BAD) ELISA Kit
ReactivityHuman
Range125-8000pg/mL
Assay Time3.5H
TargetBAD
UniProt IDQ92934
Sensitivity75 pg/mL
Alternative namesBBC2, BCL2L8
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BAD. You can calculate the concentration of Human BAD in the samples by comparing the OD of the samples to the standard curve.
Sample TypesTissue homogenate and Cell lysate
Expiration DatePlease enquire.
Human Bcl2 Associated Death Promoter (BAD) ELISA Kit

  • Human BAD ELISA Kit [orb563179]

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    0.094ng/ml

    48 T, 96 T
  • Human Bcl-xl ELISA Kit [orb405653]

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    38.7pg/mL

    48 T, 96 T