Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit

• Catalog Number: orb1736580
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 1.56-100 ng/mL
• Sensitivity: 0.59 ng/mL
• Alternative names: Anti-Tissue Transglutaminase IgG
• Note: For research use only
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Expiration Date: Please enquire.