Human Angiopoietin-like 3 / ANGPTL3 Protein, Fc Tag

• Catalog Number: orb1806471
• Category: Proteins
• Description: Angiopoietin-like protein 3 (ANGPTL3) is also known as Angiopoietin-related protein 3, Angiopoietin-5 (ANGPT5 / ANG-5), is a member of the angiopoietin-like family of secreted factors. ANGPTL3 / ANGPT5 is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. Angptl3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. Circulating PL-riched HDL particles have high cholesterol efflux abilities.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 78.2 kDa
• Target: Angiopoietin-like 3
• Source: Human Angiopoietin-like 3 Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ser 17 - Glu 460 (Accession # Q9Y5C1-1).
• Expression System: HEK293
• Expression Region: Ser 17 - Glu 460
• Endotoxins: 1.0 EU per μg
• Storage: -20°C
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, 0.05% CHAPS, pH 7.5
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 78.2 kDa. The protein migrates as 65 kDa and 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.