Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit

SKU: orb778104

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Beta(SCNN1b).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCNN1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCNN1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCNN1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCNN1b in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.111 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ENaCb ELISA Kit, ENaCbeta ELISA Kit, SCNEB ELISA Kit, Liddle Syndrome ELISA Kit, Sodium Channel,Nonvoltage-Gated 1,Beta ELISA Kit, Epithelial Na(+) channel subunit beta ELISA Kit, Nonvoltage-gated sodium channel 1 subunit beta ELISA Kit

Quick Database Links

UniProt

P51168