Human Activin A / INHBA Protein

• Catalog Number: orb16656
• Category: Proteins
• Description: Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely inhibin down regulates FSH synthesis and inhibits FSH secretion.Activins are nonglycosylated homodimers or heterodimers of various β subunits (βA, βB, βC, and βE in mammals), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a widely expressed homodimer of two βA chains. The βA subunit can also heterodimerize with a βB or βC subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human βA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat βA.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 13.0 kDa
• Target: Activin A
• Source: Human Activin A, premium grade (orb16656) is expressed from human 293 cells (HEK293). It contains AA Gly 311 - Ser 426 (Accession # AAH07858.1).
• Expression System: HEK293
• Biological Activity: Immobilized Human Activin A, premium grade at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag with a linear range of 0.4-25 ng/mL (QC tested).
• Expression Region: Gly 311 - Ser 426
• Endotoxins: 0.1 EU per μg
• NCBI: AAH07858.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 0.056% TFA in 30% ACN
• Alternative names: Activin A,INHBA
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 13.0 kDa. As a result of glycosylation, the protein migrates as 14-15 kDa under reducing (R) condition, and 23-25 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Basu, Moitri et al. Invasion of ovarian cancer cells is induced byPITX2-mediated activation of TGF-β and Activin-A Mol Cancer, 14, 162 (2015)

PubMed: 26298390

SDS-PAGE analysis of Human Activin A protein

Immobilized Human Activin A, premium grade (Cat. No. orb16656) at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. orb1101193) with a linear range of 0.4-25 ng/mL (QC tested).