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Human 5-Hydroxytryptamine Receptor 1A (5HTR1A) ELISA Kit

Catalog Number: orb778146

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SizePriceQuantity
48 T$ 300.00
96 T$ 420.00
48 T Enquire
96 T Enquire
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Catalog Numberorb778146
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 1A(5HTR1A).
ReactivityHuman
Concentration10 ng/mL
UniProt IDP08908
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR1A in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.059 ng/mL
Sample TypesTissue homogenates and other biological fluids.
Alternative names5HTR1A ELISA Kit, 5HTR1-A ELISA Kit, 5-HTR1-A ELIS
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Research AreaNeuroscience
NoteFor research use only
Expiration DatePlease enquire.
Human 5-Hydroxytryptamine Receptor 1A (5HTR1A) ELISA Kit