HTR7 antibody

• Catalog Number: orb393190
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HTR7.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 5-HT7. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HTR7
• Entrez: 65032, 3363, 15566
• UniProt ID: P32305, P34969, P32304
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: 5-hydroxytryptamine receptor 7; 5-HT-7; 5-HT7; 5-H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 5-HT7 expression in Hela (A), H1688 (B) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)

Immunofluorescent analysis of 5-HT7 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.