HSPB8/Hsp22 Antibody

• Catalog Number: orb18992
• Category: Antibodies
• Description: HSPB8/Hsp22 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196). Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 21604 MW
• UniProt ID: Q9UJY1
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Heat shock protein beta-8;HspB8;Alpha-crystallin C
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-HSPB8/Hsp22 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody.Lane 1:human MCF-7 cell; 2:human U87 cell;3:rat H9C2 cell;4:rat heart tissue;5:mouse C2C12 cell;6:mouse skeletal muscle tissue;7:mouse heart tissue.

IF analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody. HSPB8/Hsp22 was detected in immunocytochemical section of MCF7 cells.

IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody. HSPB8/Hsp22 was detected in paraffin-embedded section of mouse pancreas tissues.

IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody. HSPB8/Hsp22 was detected in paraffin-embedded section of rat pancreas tissues.

IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody. HSPB8/Hsp22 was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody. HSPB8/Hsp22 was detected in paraffin-embedded section of human mammary cancer tissues.