HSP90 alpha Mouse Monoclonal Antibody

SKU: orb500766

Description

HSP90 alpha Mouse Monoclonal Antibody

Research Area

Cancer Research, Signal Transduction, Tumor Biomarkers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B5J9
• Immunogen: Recombinant human HSP90 alpha Protein (549-692/732aa)
• Target: HSP90AA1
• Molecular Weight: 81 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; 86kDa; 89kDa; Hsp86-1; hsp4; HS90A_HUMAN; HSP90AA1; Heat shock 86 kDa (HSP 86 | HSP86); Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2 (LAP-2 | LPS-associated protein 2); Renal carcinoma antigen NY-REN-38; 3.6.4.10; HS90A_MOUSE; Tumor-specific transplantation 86 kDa antigen (TSTA); HS90A_RAT;

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (ascites of orb500766 3H7) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Sample: Hela Cell (Human) Lysate at 40 ug, NIH/3T3 Cell (Mouse) Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 98 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Rat Placenta tissue lysates, Lane 5: Human U251 cell lysates, Lane 6: Human Molt-4 cell lysates, Lane 7: Human HL60 cell lysates, Lane 8: Human A549 cell lysates, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Quick Database Links

Gene Symbol

HSP90AA1

UniProt

P07900