HSP90 alpha Mouse Monoclonal Antibody

• Catalog Number: orb500766
• Category: Antibodies
• Description: HSP90 alpha Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3H7
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human HSP90 alpha Protein (549-692/732aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 81 kDa
• Target: HSP90AA1
• UniProt ID: P07900
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HSP-90 alpha; HSP 86; HSP 86; Renal carcinoma anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (ascites of orb500766 3H7) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Sample: Hela Cell (Human) Lysate at 40 ug, NIH/3T3 Cell (Mouse) Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 98 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Rat Placenta tissue lysates, Lane 5: Human U251 cell lysates, Lane 6: Human Molt-4 cell lysates, Lane 7: Human HL60 cell lysates, Lane 8: Human A549 cell lysates, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.