HSP90 alpha Antibody

• Catalog Number: orb396189
• Category: Antibodies
• Description: Mouse Anti-Human HSP90 alpha Monoclonal IgG2a
• Target: HSP90 alpha
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide
• Purification: Protein G Purified
• Immunogen: Recombinant human HSP90alpha; Specificity mapped to amino acids 604-731
• UniProt ID: P07900
• MW: 90kDa
• Tested applications: ELISA, IHC, WB
• Dilution range: WB (1:1000), IHC (1:5000); optimal dilutions for assays should be determined by the user.
• Application notes: 1 µg/ml of SMC-108 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: Hyb-K41009
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSP86 Antibody, HSP89A Antibody, HSP90A Antibody,
• Note: For research use only
• Entrez: 3320
• NCBI: NP_001017963.2

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Western Blot analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells.