Hsp70/HSPA1A/HSPA1B Antibody

• Catalog Number: orb18121
• Category: Antibodies
• Description: Hsp70/HSPA1A/HSPA1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70 (13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 70 kDa
• UniProt ID: P0DMV9, P0DMV8
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Heat shock 70 kDa protein 1A; Heat shock 70 kDa pr
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Rat Liver Tissue Lysate, Lane 4: Rat Lung Tissue Lysate, Lane 5: CEM Cell Lysate, Lane 6: HELA Cell Lysate, Lane 7: SMMC Cell Lysate, Lane 8: HT1080 Cell L

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using HSP70s antibody.

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using HSP70s antibody.

Confocal immounofluoroscence analysis of HELA Cell using HSP70s antibody.

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using HSP70s antibody.