HSP70 (phospho-Tyr525) antibody

• Catalog Number: orb5488
• Category: Tools
• Description: HSP70 (phospho-Tyr525) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr525 (EK(p-Y)KA)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg/Test, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 70 kDa
• Target: phospho-HSP70 (Tyr525)
• UniProt ID: P0DMV8
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: HSP70 (phospho Tyr525); HSP70 (phospho Y525); p-HS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jammes, Yves et al. Fatiguing stimulation of one skeletal muscle triggers heat shock protein activation in several rat organs: the role of muscle innervation J Exp Biol, 215, 4041-4048 (2012)

PubMed: 22899526

Flow cytometric analysis of Jurkat using HSP70 (phospho-Tyr525) antibody.