Hsp27/HSPB1 Antibody

• Catalog Number: orb215991
• Category: Antibodies
• Description: Hsp27/HSPB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, - Flow Cytometry, 1-3μg/mlx106 cells, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22783 MW
• UniProt ID: P04792
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Heat shock protein beta-1;HspB1;28 kDa heat shock
• Note: For research use only
• Application notes: WB: The detection limit for Hsp27 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-HSP27 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of HSP27 using anti-HSP27 antibody.Lane 1:human A549 cell; 2:human CACO-2 cell; 3:human HeLa cell; 4:human MCF-7 cell; 5:human HT1080 cell.

IF analysis of HSP27 using anti-HSP27 antibody.HSP27 was detected in immunocytochemical section of U20S cells.

IHC analysis of HSP27 using anti-HSP27 antibody.HSP27 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of HSP27 using anti-HSP27 antibody.HSP27 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of HSP27 using anti-HSP27 antibody.HSP27 was detected in frozen section of human placenta tissue.