HSP27 (phospho-Ser78) antibody

• Catalog Number: orb5481
• Category: Antibodies
• Description: HSP27 (phospho-Ser78) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP27 around the phosphorylation site of Ser78 (AL(p-S)RQ)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=0.2μg /test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: Phospho-HSP27 (Ser78)
• UniProt ID: P04792
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Hsp27 (phospho S78); p-Hsp27 (phospho S78); Heat s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ICC analysis of Hela cell using Phospho-HSP27 (Ser78) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of Rat heart using Phospho-HSP27 Ser78 Polyclonal Antibody at 1:400 dilution.

Western blot:Lane 1:MCF-7 (Human) Cell;Lane 2:U251 (Human) Cell;Lane 3:U2os (Human) Cell;Lane 4:A431 (Human) Cell;Lane 5:Huvec (Human) Cell;Lane 6:A549 (Human) Cell;Primary:Anti-Phospho-HSP27 (Ser78) at 1:1000 dilution.

Western blot:Muscle (Mouse);Primary:Anti-Phospho-HSP27(Ser78) at 1:300 dilution.