HSP22 Mouse Monoclonal Antibody

SKU: orb500003

Description

HSP22 Mouse Monoclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4C3
• Immunogen: Recombinant human HSP22 Protein
• Target: HSPB8
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMT2L; DHMN2; E2IG1; H11; HMN2; HMN2A; HMND2; HSP22; HSPB8-N1; HSPB8-N2; MFM13; Cryac; D5Ucla4; H11K; HSP20-like; HSPB8_HUMAN; HSPB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Heat shock protein family B member 8; Protein kinase H11; Small stress protein-like protein HSP22; HSPB8_MOUSE; HSPB8_RAT;

Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with HSP22 Monoclonal Antibody, Unconjugated (orb500003) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human 293T cell lysates, Lane 5: Human U-2 OS cell lysates, Primary: Anti-HSP22 (orb500003) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 26 kDa.

Quick Database Links

Gene Symbol

HSPB8

UniProt

Q9UJY1