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Catalog Number | orb402576 |
---|---|
Category | Antibodies |
Description | Hsp20/HSPB6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Hsp20 recombinant protein (Position: H73-L123). Human Hsp20 shares 88.2% and 92.2% amino acid (aa) sequence identity with mouse and rat Hsp20, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 17136 MW |
UniProt ID | O14558 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Heat shock protein beta-6;HspB6;Heat shock 20 kDa- Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of Hsp20 using anti-Hsp20 antibody.Lane 1:rat cardiac muscle Tissue;2:rat skeletal muscle Tissue;3:rat testis Tissue;4:mouse testis Tissue;5:human A431 Cell.
IF analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in immunocytochemical section of A431 cells.
IHC analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in paraffin-embedded section of human skeletal muscle tissue.
IHC analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in paraffin-embedded section of human mammary cancer tissue.
IHC analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in paraffin-embedded section of mouse lung tissue.
IHC analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in paraffin-embedded section of mouse cardiac muscle tissue.
IHC analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in paraffin-embedded section of rat lung tissue.
IHC analysis of Hsp20 using anti-Hsp20 antibody. Hsp20 was detected in paraffin-embedded section of rat cardiac muscle tissue.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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