Hsp105/HSPH1 Antibody

• Catalog Number: orb443112
• Category: Antibodies
• Description: Hsp105/HSPH1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Hsp105 recombinant protein (Position: Y653-D858).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 105 kDa
• UniProt ID: Q92598
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Heat shock protein 105 kDa; Antigen NY-CO-25; Heat
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-Hsp105 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Hsp105 using anti-Hsp105 antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human COLO-320 cell;4:human SGC-7901 cell;5:human HepG2 cell;6:human K562 cell;7:human Jurkat cell;8:human SK-OV-3 cell.

WB analysis of Hsp105 using anti-Hsp105 antibody.Lane 1:rat brain tissue;2:rat lung tissue;3:mouse brain tissue;4:mouse lung tissue;5:mouse NIH3T3 cell.

IF analysis of Hsp105 using anti-Hsp105 antibody.Hsp105 was detected in immunocytochemical section of U20S cells.

IHC analysis of Hsp105 using anti-Hsp105 antibody.Hsp105 was detected in paraffin-embedded section of human testis tissues.

IHC analysis of Hsp105 using anti-Hsp105 antibody.Hsp105 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of Hsp105 using anti-Hsp105 antibody.Hsp105 was detected in paraffin-embedded section of human intestinal cancer tissues.