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Catalog Number | orb2292773 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant HSD3B1. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1 Kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Protein Sequence | MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ |
Tested applications | ELISA, IHC-P, WB |
Clone Number | 3C11-D4 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | AAH31999.1 |
HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Western Blot analysis of HSD3B1 expression in human placenta.
Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification. [antibody concentration 3 ug/ml](a) Stomach(b) Esophagus(c) Endometrium(d) Uterine cervix(e) Placenta(f) Ovary, clear cell carcinoma(g) Hepatocellular carcinoma(h) Breast cancer(i) Colon adenocarcinoma (j and k) Cervical carcinoma(l, m, and n) Choriocarcinoma(o) Epithelioid trophoblastic tumor.
Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (66.77 KDa).