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    HSD17B4 antibody

    Catalog Number: orb234876

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb234876
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to HSD17B4
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Primate, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSD17B4. The exact sequence is proprietary.
    Dilution rangeWB: 1:500-1000
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetHSD17B4
    Entrez15488, 79244, 3295
    UniProt IDP51660, P51659, P97852
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti EDH17B4 antibody, anti Peroxisomal multifunct
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    HSD17B4 antibody

    Western blot analysis of HSD17B4 expression in HEK293T (A), H1688 (B), H1792 (C), mouse liver (D), rat liver (E) whole cell lysates. (Predicted band size: 79 kD; Observed band size: 90 kD)

    HSD17B4 antibody

    Immunohistochemical analysis of HSD17B4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    HSD17B4 antibody

    Immunofluorescent analysis of HSD17B4 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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