HSD17B1 Antibody

• Catalog Number: orb137855
• Category: Antibodies
• Description: HSD17B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1 (29-43aa QSFKVYATLRDLKTQ), different from the related rat and mouse sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 34950 MW
• UniProt ID: P14061
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Estradiol 17-beta-dehydrogenase 1;1.1.1.62;17-beta
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

References

Yang, Yan et al. Directed mouse embryonic stem cells into leydig-like cells rescue testosterone-deficient male rats in vivo Stem Cells Dev, 24, 459-470 (2015)

PubMed: 25340537

Applications

IHC

Reactivity

Rat

Kozlowska, Wiktoria et al. Electromagnetic field exposure alters in vitro estrogen biosynthesis and its release by the porcine endometrium in the peri-implantation period Reprod Biol, 22, 100642 (2022)

PubMed: 35487179

Applications

WB

Reactivity

Porcine

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Ovary Tissue using HSD17B1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Placenta Tissue using HSD17B1 antibody

Confocal immunofluoroscence analysis of HELA cells using HSD17B1 antibody

Western blot analysis of Lane 1: Rat Kidney Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: 293T Cell Lysate, Lane 4: HELA Cell Lysate using HSD17B1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Liver Tissue using HSD17B1 antibody