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Catalog Number | orb390605 |
---|---|
Category | Tools |
Description | Detect mature miRNA hsa-mir-3164 expression normalized by cel-mir-39-3p. |
Tested applications | PCR |
Target | hsa-mir-3164 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
Sensitivity | 10 copies of a standard mimics and as little as 25 pg of total RNA |
Storage | Shipped at 4°C. Store at -20°C for 6 months. |
Note | For research use only |
Application notes | The Real-Time RT-PCR detection and cel-mir-39-3p calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and cel-mir-39-3p primer set for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. < br>< strong>Experiment Notes: < b>PCR Method: 2-Step qRT-PCR < b>Detection Range: Seven orders of magnitude < b>Sample Types: miRNA, Total RNAPlatform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480Components: < br>50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions. 100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions. 200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions. |
Expiration Date | 12 months from date of receipt. |
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