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Catalog Number | orb1246538 |
---|---|
Category | Antibodies |
Description | HRASLS2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: QELPYSLTSDNC |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 21kDa band in Human Kidney lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.4kDa according to NP_060348.1 (provisional). The 21kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | HRASLS2 |
UniProt ID | Q9NWW9 |
NCBI | NP_060348.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | HRASLS2 , HRAS-like suppressor 2, FLJ20556 Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 21kDa band in Human Kidney lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.4kDa according to NP_060348.1 (provisional). The 21kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246538 (5 ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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