HPV16 E7 antibody

• Catalog Number: orb10573
• Category: Tools
• Description: HPV16 E7 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from HPV16 E7 protein (41-90/98 aa)
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1ug/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: HPV16 E7
• UniProt ID: P03129
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: E7; HPV16 E7 protein; HPV 16 E7 protein; Human Pap
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hu, Zheng et al. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy J Clin Invest, 125, 425-436 (2015)

PubMed: 25500889

Applications

IHC, WB

Reactivity

Mouse

Gao, Chun et al. The application of CRISPR/Cas9 system in cervical carcinogenesis Cancer Gene Ther, (2021)

PubMed: 34349239

Applications

IF, IHC, WB

Reactivity

Mouse

Xiong, Jinfeng et al. E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester) Int J Nanomedicine, 16, 7609-7622 (2021)

PubMed: 34819726

Applications

IHC

Reactivity

Mouse

Zhu, Da et al. Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy Mol. Ther., 26, 2443-2455 (2018)

PubMed: 30241742

Applications

IF, IHC

Reactivity

Mouse

Li, Mengtao et al. Exposure to microbial metabolite butyrate prolongs the survival time and changes the growth pattern of HPV16 E6/E7-immortalized keratinocytes in vivo Am J Pathol, (2021)

PubMed: 34214507

Applications

IF

Reactivity

Mouse

Immunohistochemical analysis of paraffin-embedded human uterus tissue using HPV16 E7 antibody

IHC-P of human uterus tissue (HPV16 E7 antibody at 1:250)

Immunohistochemical analysis of formalin fixed and paraffin embedded human breast cancer using HPV16 E7 antibody

Representative data from H&E staining and E7 IHC staining at treatment days 0, 12, 18, and 24. The mice were treated with HPV16-E7-T512 using the 3-day interval regimen. n = 3 per group. Scale bars: 80 um.