HP1 gamma Mouse Monoclonal Antibody

SKU: orb500701

Description

HP1 gamma Mouse Monoclonal Antibody

Research Area

DNA and RNA, Methyl lysine, Methylated DNA

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B8H6
• Immunogen: Recombinant human HP1 gamma Protein full length
• Target: CBX3
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HECH; HP1-GAMMA; HP1Hs-gamma; HP1gamma; HP1g; M32; CBX3_HUMAN; CBX3; Heterochromatin protein 1 homolog gamma (HP1 gamma); Modifier 2 protein; CBX3_MOUSE;

Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (cell supernatant of orb500701) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (cell supernatant of orb500701) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Molt-4 cell (human) Lysate at 30 ug, jurkat cell (human) Lysate at 30 ug, HepG2 cell (human) Lysate at 30 ug, Primary: Anti-HP1 gamma (orb500701) at 1/5000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Quick Database Links

Gene Symbol

CBX3

UniProt

Q13185