HP Antibody

• Catalog Number: orb1262448
• Category: Antibodies
• Description: HP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-322 amino acids from the Central region of human HP.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: HP
• UniProt ID: P00738
• NCBI: P00738
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Haptoglobin, Zonulin, Haptoglobin alpha chain, Hap
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:8000 dilution + Human liver lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of HP using rabbit polyclonal HP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HP gene (Lane 2).

Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with HP Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.