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Catalog Number | orb215972 |
---|---|
Category | Antibodies |
Description | HOXA11 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human HOXA11 recombinant protein (Position: H74-R245). Human HOXA11 shares 95% amino acid (aa) sequence identity with mouse HOXA11. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 34486 MW |
UniProt ID | P31270 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Homeobox protein Hox-A11;Homeobox protein Hox-1I;H Read more... |
Note | For research use only |
Application notes | WB: The detection limit for HOXA11 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-HOXA11 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of HOXA11 using anti-HOXA11 antibody.Lane 1:Recombinant Human HOXA11 Protein 0.5ng.
WB analysis of HOXA11 using anti-HOXA11 antibody.Lane 1:Rat Brain Tissue;2:Human Placenta Tissue;3:HLEA Cell;4:HT1080 Cell;5:HEPA Cell.
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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