Horse TNNI3(Troponin I Type 3, Cardiac) ELISA Kit

• Catalog Number: orb1146876
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 3, Cardiac(TNNI3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Troponin I Type 3, Cardiac(TNNI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 3, Cardiac(TNNI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 3, Cardiac(TNNI3) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Equine
• Range: 31.25-2000 pg/mL
• UniProt ID: Q5PYI0
• Sensitivity: 11.9 pg/mL
• Protein Length: 3.5h
• Alternative names: cTnI, TNNC1, CMH7, C-TnI, Troponin I Type 3, Cardi
• Note: For research use only
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.