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Catalog Number | orb347143 |
---|---|
Category | Antibodies |
Description | Horse IgG (H&L) antibody (Alkaline Phosphatase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Equine |
Isotype | IgG |
Immunogen | Horse IgG whole molecule |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG and Horse Serum. |
Conjugation | AP |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat Anti-Horse IgG alkaline phosphatase Conjugate Read more... |
Note | For research use only |
Application notes | Anti-Horse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC, WB | |
Equine | |
Rabbit | |
Polyclonal | |
AP |
ELISA, IHC, WB | |
Equine | |
Rabbit | |
Polyclonal | |
AP |
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