Horse IgG F(c) antibody

Catalog Number: orb347135

Catalog Number	orb347135
Category	Antibodies
Description	Horse IgG F(c) antibody
Species/Host	Goat
Clonality	Polyclonal
Tested applications	DOT, ELISA, IHC, WB
Reactivity	Equine
Isotype	IgG
Immunogen	Horse IgG F(c) fragment
Concentration	2.3 mg/mL
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/Appearance	Liquid (sterile filtered)
Purity	Anti-Horse IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab).
Conjugation	Unconjugated
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Alternative names	goat Anti-Horse IgG F(c) Antibody, goat Anti-Horse Read more...
Note	For research use only
Application notes	Anti-Horse IgG F(c) antibody has been tested by ELISA and dot blot and is suitable for Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.
Expiration Date	12 months from date of receipt.

Applications:	ELISA, IHC, SDS-PAGE, WB
Conjugation:	Unconjugated
Purity:	Horse IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain.
Source:	Horse

Sizes Available: 1 mg

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Horse IgG, anti-Horse IgG F(c) and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain.
Source:	Horse

Sizes Available: 1 mg

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Horse IgG, anti-Horse IgG F(c) and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain.
Source:	Horse

Sizes Available: 1 mg

Conjugation:	Biotin
Purity:	This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Horse IgG, anti-Horse IgG F(c) and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain.
Source:	Horse

Sizes Available: 1 mg