Horse IgG Fc Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347165
• Category: Antibodies
• Description: Horse IgG F(c) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Equine
• Isotype: IgG
• Immunogen: Horse IgG F(c) fragment
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Horse IgG F(c) Antibody Alkaline Phosp
• Note: For research use only
• Application notes: Anti-Horse IgG F(c) Alkaline Phosphatase has been tested by ELISA. This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Purified Rabbit Anti-Horse IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Horse IgG F(c). Each well was coated in duplicate with 1.0 µg of Horse IgG F(c) (p/n orb346177) [Red Line] and Horse IgG (p/n orb2652757) [Green Line]. The working dilution of Horse IgG F(c) Alk Phos is 1:2400. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using NPP ELISA Alk Phos Substrate, NPP Buffer, and Horse IgG F(c) Alk Phos.