HOPX/LAGY Rabbit Polyclonal Antibody

SKU: orb1358

Description

HOPX/LAGY Rabbit Polyclonal Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HOPX/LAGY (21-60/73aa)
• Target: HOPX
• Molecular Weight: 8 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HOP; LAGY; CAMEO; HOD; NECC1; OB1; SMAP31; TOTO; 1110018K11Rik; 1200015P04Rik; 2300002F06Rik; Hdop; Obl; GIIg15b; HOP_BOVIN; HOPX; Odd homeobox protein 1; HOP_HUMAN; Lung cancer-associated Y protein; Not expressed in choriocarcinoma protein 1; HOP_MOUSE; Homeobox-only protein; Odd homeobox protein 1 (mOB1); HOP_PIG; HOP_RAT; Global ischemia-induced gene 15B protein (GIIg15b);

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-LAGY/AF488 antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HOPX) Polyclonal Antibody, Unconjugated (orb1358) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

HOPX

UniProt

Q9BPY8