You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb546389 |
---|---|
Category | Antibodies |
Description | HOOK2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human HOOK2 recombinant protein (Position: E500-R702). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 83 kDa |
UniProt ID | Q96ED9 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein Hook homolog 2; h-hook2; hHK2; HOOK2 Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-HOOK2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U87 cells using anti-HOOK2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of HOOK2 using anti-HOOK2 antibody.Lane 1:human RT4 cell;2:human MCF-7 cell.
IF analysis of HOOK2 using anti-HOOK2 antibody. HOOK2 was detected in immunocytochemical section of A431 cells.
IHC analysis of HOOK2 using anti-HOOK2 antibody. HOOK2 was detected in a paraffin-embedded section of human ovarian cancer tissue.
IHC analysis of HOOK2 using anti-HOOK2 antibody. HOOK2 was detected in a paraffin-embedded section of human placenta tissue.
Filter by Rating