hnRNPL antibody

• Catalog Number: orb76673
• Category: Antibodies
• Description: Mouse monoclonal antibody to hnRNPL
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4D11
• Tested applications: ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Amphibian, Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose
• Concentration: 1mg/ml
• Dilution range: Optimal antibody dilution should be determined by titration, however as a guideline try; IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ICC/IF: 1:100 on paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. WB: (0.001µg/ml) staining in Jurkat and HepG2 cells nuclear lysate. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).Flow Cyt: Use 1µg for 106 cells.
• Conjugation: Unconjugated
• Target: hnRNP L
• Entrez: 80846, 15388, 3191
• UniProt ID: P31943
• Buffer/Preservatives: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
• Note: For research use only
• Application notes: SP2/0
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Hela extracts cell lysate using hnRNPL antibody