hnRNP M3/4 Antibody

• Catalog Number: orb1523949
• Category: Antibodies
• Description: hnRNP M3/4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 475 and 525
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: hnRNP M3/4
• UniProt ID: P52272
• NCBI: NP_005959.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HNRPM; NAGR1; N-acetylglucosamine receptor 1; HTGR
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , Jurkat (50µg) , and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP M3/4 antibody orb1523949 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human hnRNP M3/4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hnRNP M3/4 antibody orb1523949 used for IP at 6 µg/mg lysate.

Detection of human hnRNP M3/4 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-hnRNP M3/4 (Cat. No. orb1523949) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse hnRNP M3/4 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-hnRNP M3/4 (Cat. No. orb1523949) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB