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Catalog Number | orb389414 |
---|---|
Category | Antibodies |
Description | HnRNP F/HNRNPF Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F (23-53aa SVEDVQNFLSDCTIHDGAAGVHFIYTREGRQ), different from the related mouse and rat sequences by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 45672 MW |
UniProt ID | P52597 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Heterogeneous nuclear ribonucleoprotein F;hnRNP F; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U87 cells using anti-HnRNPF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of HnRNPF using anti-HnRNPF antibody.Lane 1:rat liver Tissue;2:mouse spleen Tissue;3:HELA cell.
IF analysis of HnRNPF using anti-HnRNPF antibody.HnRNPF was detected in immunocytochemical section of SKOV-3 cells.
IHC analysis of HnRNPF using anti-HnRNPF antibody.HnRNPF was detected in paraffin-embedded section of human intetsinal cancer tissues.
IHC analysis of HnRNPF using anti-HnRNPF antibody.HnRNPF was detected in paraffin-embedded section of human mammary cancer tissues.
IHC analysis of HnRNPF using anti-HnRNPF antibody.HnRNPF was detected in paraffin-embedded section of mouse intestine tissues.
IHC analysis of HnRNPF using anti-HnRNPF antibody.HnRNPF was detected in paraffin-embedded section of rat intestine tissues.
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC-Fr, IHC-P, WB | |
Canine, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Biotin |
IHC-P | |
Canine, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-Fr, IHC-P, WB | |
Canine, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
HRP |
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