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Description
Research Area
Neuroscience
Images & Validation
−| Tested Applications | ELISA, FC, ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try;Flow Cyt: Use 1µg for 106 cells.IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocolWB: Use at 1:2000-1:4000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa)IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 9H10 |
| Immunogen | Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human) |
| Target | hnRNP A1 |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HNRNPA 1 antibody;Helix destabilizing protein antibody;Helix-destabilizing proteinantibody;Heterogeneous nuclear ribonucleoprotein A1 antibody;Heterogeneous nuclear ribonucleoprotein A1B proteinantibody;Heterogeneous nuclear ribonucleoprotein B2 protein antibody;Heterogeneous nuclear ribonucleoprotein coreprotein A1 antibody;hnRNP A1 antibody;hnRNP core protein A1 antibody;HNRNPA1 antibody;HNRPA1antibody;MGC102835 antibody;Nuclear ribonucleoprotein particle A1 protein antibody;ROA1_HUMANantibody
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation





