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hnRNP A1 Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, FC, ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try;Flow Cyt: Use 1µg for 106 cells.IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocolWB: Use at 1:2000-1:4000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa)IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 9H10 |
| Immunogen | Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human) |
| Target | hnRNP A1 |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded [9H10] staining human lung using hnRNPA1 antibody
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Protocol Information
hnRNP A1 Monoclonal Antibody (orb76660)
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