HNF1B antibody

• Catalog Number: orb19402
• Category: Antibodies
• Description: Goat polyclonal antibody to HNF1B
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat, Zebrafish
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 61.3; 58.4
• Target: TCF2 / VHNF1
• Entrez: 6928
• Protein Sequence: QAYDRQKNPSKEER
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TCF2 antibody, anti VHNF1 antibody, anti tran
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19402 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb19402 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.