HMOX2 Antibody

• Catalog Number: orb1249527
• Category: Antibodies
• Description: HMOX2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DQAGSTLARETLED
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of cell line K562 (calculated MW of 36.0kDa according to NP_002125.3). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Pancreas cancer shows increased staining of invading cancer cells. Recommended concentration, 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HMOX2
• UniProt ID: P30519
• NCBI: NP_002125.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HMOX2, heme oxygenase (decycling) 2, HO-2, heme ox
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of cell line K562 (calculated MW of 36.0kDa according to NP_002125.3). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Pancreas cancer shows increased staining of invading cancer cells. Recommended concentration, 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249527 (0.3 ug/ml) staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249527 (2 ug/ml) staining of paraffin embedded Human Pancreas: cancer (left), adjacent to cancer (right). Steamed antigen retrieval with citrate buffer pH 6, Alexa594-staining.