HMGXB3 antibody

• Catalog Number: orb665862
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HMGXB3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HMGXB3. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HMGXB3
• Entrez: 22993
• UniProt ID: Q12766
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-KIAA0194 antibody, anti-SMF antibody, anti-HM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HMGXB3 expression in A549 (A), MCF7 (B) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 168 kD)

Immunohistochemical analysis of HMGXB3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.