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Catalog Number | orb371736 |
---|---|
Category | Antibodies |
Description | HMGB2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB2 (65-97aa KSDKARYDREMKNYVPPKGDKKGKKKDPNAPKR), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunofluorescence, 5μg/ml, Rat Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 24034 MW |
UniProt ID | P26583 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | High mobility group protein B2;High mobility group Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-HMGB2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of HMGB2 using anti-HMGB2 antibody.Lane 1:human Jurkat cell;2:rat PC-12 cell;3:mouse spleen tissue.
IF analysis of HMGB2 using anti-HMGB2 antibody.HMGB2 was detected in immunocytochemical section of U20S cells.
IF analysis of HMGB2 and Tubulin alpha using anti-HMGB2 antibody and anti-Tubulin alpha antibody. HMGB2 and Tubulin alpha were detected in immunocytochemical section of MCF-7 cells.
IF analysis of HMGB2 using anti-HMGB2 antibody. HMGB2 was detected in a paraffin-embedded section of rat intestine tissue.
IHC analysis of HMGB2 using anti-HMGB2 antibody. HMGB2 was detected in a paraffin-embedded section of mouse intestine tissue.
IHC analysis of HMGB2 using anti-HMGB2 antibody. HMGB2 was detected in a paraffin-embedded section of rat spleen tissue.
IHC analysis of HMGB2 using anti-HMGB2 antibody. HMGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue.
ICC, IHC-Fr, IHC-P, IP, WB | |
Hamster, Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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