HMGB1 (AcK12) antibody

• Catalog Number: orb339062
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HMGB1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat
• Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K12 of human HMGB1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HMGB1
• Entrez: 25459, 100862258, 15289, 3146
• UniProt ID: P63158, P09429, P63159
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HMG1 antibody, anti High mobility group prote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HMGB1 (Acetyl-K12) expression in DLD (A), rat liver (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Direct ELISA antibody dose-response curve using Anti-HMGB1 (Acetyl-K12) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.