HLA-G antibody (PE)

• Catalog Number: orb43619
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: 5A6G7
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of HLA-G transfected HEK-293 cells stained using anti-human HLA-G (5A6G7) PE antibody (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from HLA-G transfected HEK-293 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 1.7 µg/ml, same as HLA-G PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HLA-G transfected HEK-293 cells suspension.