HLA-G antibody (FITC)

• Catalog Number: orb43626
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: 87G
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line.
• Expiration Date: 12 months from date of receipt.

Separation of cells stained using anti-human HLA-G (87G) FITC antibody (concentration in sample 5 μg/ml, red-filled) from cells stained using mouse IgG2a isotype control (MOPC-173) FITC antibody (concentration in sample 5 μg/ml, same as HLA-G FITC concentration, black-dashed) in flow cytometry analysis (surface staining) of HLA-G transfected cells.